In Vitro:Article Title: Simplicilones A and B Isolated from the Endophytic Fungus Simplicillium subtropicum SPC3
Article Snippet: .. In vitro cytotoxicity (IC 50 ) of the pure compounds was investigated against the established mouse fibroblast cell line L929 (DSMZ no. ACC 2) and KB3.1 using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method in 96-well microplates for tissue cultures. .. The cell line L929 was cultured in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, Basel, Switzerland), supplemented with 10% foetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and incubated under 10% CO 2 at 37 °C for 5 days.
MTT Assay:Article Title: Simplicilones A and B Isolated from the Endophytic Fungus Simplicillium subtropicum SPC3
Article Snippet: .. In vitro cytotoxicity (IC 50 ) of the pure compounds was investigated against the established mouse fibroblast cell line L929 (DSMZ no. ACC 2) and KB3.1 using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method in 96-well microplates for tissue cultures. .. The cell line L929 was cultured in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, Basel, Switzerland), supplemented with 10% foetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and incubated under 10% CO 2 at 37 °C for 5 days.
Isolation:Article Title: A Fast and Reliable Process to Fabricate Regenerated Silk Fibroin Solution from Degummed Silk in 4 Hours
Article Snippet: EDX spectra and elemental maps were obtained by acquiring area scans using a Zeiss DSM 950 scanning electron microscope (Carl Zeiss, Jena, Germany) with a Noran System 7 EDX with an SDD detector (Thermo Fisher Scientific, Waltham, MA, USA). .. Possible cytotoxic effects of the silk fibroin isolated with ZnCl 2 were analyzed by a direct cell contact assay using the mouse fibroblast cell line L929 (German Collection of Microorganisms and Cell Cultures, DSMZ, Braunschweig, Germany). .. L929 cells were cultured in Roswell Park Memorial Institute 1640 (RPMI 1640) medium (Lonza, Basel, Switzerland) containing 10% fetal bovine serum (Fisher Scientific, Schwerte, Germany), 2 mM Glutamax (Lonza, Basel, Switzerland) and 1.0% ( v / v ) penicillin/streptomycin (Lonza, Basel, Switzerland).
Cell Culture:Article Title: Cytocompatibility Assessment of L-PBF-Manufactured Zinc–Silver–Copper Alloys for Customized Biodegradable Medical Implants
Article Snippet: .. To test the biocompatibility, the mouse fibroblast cell line L929 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany), cultured in Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS SUPERIOR, Bio&SELL GmbH, Feucht, Germany) and 1% L-glutamine (Thermo Fisher Scientific Inc.) was used. .. In addition, the human primary osteogenic sarcoma cell line SAOS-2 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), cultured in McCoy’s 5A medium (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) supplemented with 15% FBS (Bio&SELL GmbH) and 1% L-glutamine (Thermo Fisher Scientific Inc.), was used.
Article Title: Cytocompatibility Assessment of L-PBF-Manufactured Zinc-Silver-Copper Alloys for Customized Biodegradable Medical Implants.
Article Snippet: .. Cultivation of Cells To test the biocompatibility, the mouse fibroblast cell line L929 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany), cultured in Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS SUPERIOR, Bio&SELL GmbH, Feucht, Germany) and 1% L-glutamine (Thermo Fisher Scientific Inc.) https://doi.org/10.3390/jfb17030146 was used. .. In addition, the human primary osteogenic sarcoma cell line SAOS-2 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), cultured in McCoy’s 5A medium (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) supplemented with 15% FBS (Bio&SELL GmbH) and 1% L-glutamine (Thermo Fisher Scientific Inc.), was used.
Modification:Article Title: Cytocompatibility Assessment of L-PBF-Manufactured Zinc–Silver–Copper Alloys for Customized Biodegradable Medical Implants
Article Snippet: .. To test the biocompatibility, the mouse fibroblast cell line L929 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany), cultured in Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS SUPERIOR, Bio&SELL GmbH, Feucht, Germany) and 1% L-glutamine (Thermo Fisher Scientific Inc.) was used. .. In addition, the human primary osteogenic sarcoma cell line SAOS-2 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), cultured in McCoy’s 5A medium (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) supplemented with 15% FBS (Bio&SELL GmbH) and 1% L-glutamine (Thermo Fisher Scientific Inc.), was used.
Article Title: Cytocompatibility Assessment of L-PBF-Manufactured Zinc-Silver-Copper Alloys for Customized Biodegradable Medical Implants.
Article Snippet: .. Cultivation of Cells To test the biocompatibility, the mouse fibroblast cell line L929 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany), cultured in Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS SUPERIOR, Bio&SELL GmbH, Feucht, Germany) and 1% L-glutamine (Thermo Fisher Scientific Inc.) https://doi.org/10.3390/jfb17030146 was used. .. In addition, the human primary osteogenic sarcoma cell line SAOS-2 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), cultured in McCoy’s 5A medium (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) supplemented with 15% FBS (Bio&SELL GmbH) and 1% L-glutamine (Thermo Fisher Scientific Inc.), was used.
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